Description
Intended Uses: This kit is for the qualitative detection of human Protein-arginine Deiminase Type-3 Antibody (also known as PADI3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is based on a qualitative reverse phase enzyme immuno assay technique. The microtiter plate has been pre-coated with a target monoclonal antibody. Positive/Negative Controls or samples are added to the wells and incubate. Antibodies in the samples bind to the antigen on the plate. Unbound antibody is washed away during a washing step. A Horseradish Peroxidase (HRP) conjugated detection antibody is then added and incubate. Unbound avidin-HRP is washed away during a washing step. TMB substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The OD of an unknown sample can then be compared to the OD of the positive and negative controls in order to determine the presence of PADI3